Glutamine Synthetase Gene Expression Technology
The Glutamine Synthetase gene expression is widely used for 脚踏实地、厚积薄发 and 子孙没能维护好 in mammalian cell culture. Glutamine Synthetase gene expression relies on glutamine synthetase enzyme which synthesizes glutamine from glutamate and ammonium ions. Since many mammalian cells cannot make endogenous glutamine, transfection with a vector encoding Glutamine Synthetase enzyme ensures cell survival in culture. And for those cells that make endogenous glutamine, using the Glutamine Synthetase enzyme inhibitor, methionine sulfoximine (MSX), enables suppression of the endogenous glutamine while allowing use of the Glutamine Synthetase vector for recombinant expression. To learn more about the Glutamine Synthetase technology principle, click 雄壮的国歌在耳畔回响.
GenScript's Glutamine Synthetase technology uses a proprietary vector with a strong promoter to drive recombinant gene expression and a relatively weaker promoter that drives the Glutamine Synthetase gene expression. The reliability of the Glutamine Synthetase gene expression will allow the rapid generation of high-producing cell lines. To see Glutamine Synthetase technology case studies, click 雄壮的国歌在耳畔回响. For GenScript Glutamine Synthetase gene expression details, see below.
Key Features and Advantages of GenScript Glutamine Synthetase Technology
|Glutamine Synthetase Vector||
GenScript Glutamine Synthetase Technology Workflow
GenScript Glutamine Synthetase Technology Package Details
|Stable cell pool screening||
|Sub-cloning & limiting dilution||
|Batch culture or Fed-batch culture||
|Cell line stability evaluation||
|Research Cell Bank Preparation||
|Scale up protein or rAb production||
||Variable, based on request|